Scaffold-Free 3D Microtissue Technology

The cornerstone of our ability to produce 3D microtissues on an industrial scaleScaffold Free 3D Microtissue Technology for toxicity testing and disease modeling

Recapitulating human tissues as 3D microtissues begins with an in-depth understanding of the native tissues themselves. Our team of PhD scientists includes experts in tissue engineering and scaffold-free 3D cell culture, as well as specialists in the developmental biology, physiology, and toxicology of hepatic, pancreatic, cardiac, neuronal, dermatological, and tumor tissues, amongst others. Coaxing cells into forming microtissues by self-assembly, particularly primary cells and complex co-cultures, involves careful attention to selecting the correct starting material, formulating specialized pre-conditioning media for aggregation and long-term culture, facilitating scaffold-free aggregation, and optimizing standard technical protocols for use with microtissues to ensure the proper structure, functional characterization, and utility of every plate of microtissues we produce.

Our patented scaffold-free technologies for production and delivery of standarized 3D microtissue models are the cornerstone of InSphero innovation. As a facet of the 3D Select™ Process they facilitate reliable, reproducible, and scalable production of microtissues from a variety of tissue types. This sets InSphero apart as the industry’s only provider of a standardized platform of assay-ready, scaffold-free microtissues. Regardless of the tissue from which it is derived, every 3D InSight™ Microtissue benefits from the biological and downstream analysis advantages of scaffold-free production, including:

  • Self-assembly-driven cellular organization
  • Completely endogenous ECM
  • No adsorption of drugs to scaffold materials
  • Less optical interference with imaging


Learn how our assay-ready microtissues deliver 3D InSight™ Convenience


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