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Akura™ 384 Spheroid Microplate
The Akura™ 384 Spheroid Microplate is our ultimate spheroid screening plate unifying robust liquid and spheroid handling with automated high-throughput screening applications. The flat bottom and black walls of the InSphero 384-well plate facilitate fluorescent imaging, while the special well design allows medium exchange, dosing, and endpoint determination in the same plate saving time and resources.
- All-in-one plate allowing spheroid formation, long-term culturing and analysis in one single plate
- Unique SureXchange™ ledge engineered to prevent unintended spheroid/organoid aspiration and to retain consistent minimal residual volume (2-3 µl) during medium exchange
- Spheroid/organoid morphology and functionality are preserved in long-term cell culture
- Superior fluorescent imaging quality with a continuous, flat-bottom, 125 μm Polystyrene membrane, and a black-walled body
Architecture
Handling
Imaging
Architecture
- The unique well design of the SureXchange™ ledge protects the spheroid by leaving a minimal amount of residual volume (2-3 μl)
- ANSI/SLAS standard format for full compatibility with automated liquid handling and high-content imaging systems
- A two-component plate consisting of a black polystyrene body and a 125 μm transparent bottom membrane
Handling
- >90% medium removal without spheroid loss using manual and automated pipetting
- Ultra-low attachment surface with a stable cell-repellent coating
- Biologically inert, and non-degradable surface compatible with cell culture and in-plate post-culture processing steps such as cell lysis and fixation procedures
Imaging
- Continuous flat bottom with excellent planarity for imaging applications with immersion objectives, minimal optical aberration, and constant spheroid z-position
- The black-walled body eliminates fluorescent crosstalk between wells
- Defined 1-mm quick-reference region of interest for increased imaging speed
Compare Akura™ 384 Spheroid Microplate and Akura™ 384 ImagePro
- Both Akura™ 384 Plates have black walls to minimize cross-talk and a unique well design for near-complete medium exchange without spheroid loss.
- The Akura™ 384 Spheroid Microplate has a 125 µm thin Polystyrene membrane. The plate is especially suited for high throughput applications, lytic and biochemical assays, and basic confocal imaging endpoints.
- The Akura™ 384 ImagePro has a flat, ultrathin, 25 µm gas-permeable membrane made of FEP (fluorinated ethylene propylene) to minimize RI mismatch and it is compatible with high NA objectives.
If you require a high-resolution, high-content imaging endpoint, we recommend the Akura™ 384 ImagePro Plate.
Product Documents
- Product Manual Akura™ 384 Spheroid Microplate
- Technical Specifications Akura™ 384 Spheroid Microplate
- Quick Start Guide Akura™ 384 Spheroid Microplate
- Technical Protocol - 3D Aggregation of Tumor Spheroids in the Akura™ 384 Spheroid Microplate
- FAQs Akura™ 384 Spheroid Microplate
- Certificate of Compliance
