Increase translational relevance with physiologically relevant tumor models
3D-cell-based assays can be a powerful tool in oncology drug discovery. However, their predictive value depends on how closely a model reflects the in vivo tumor microenvironment and cancer progression. 3D InSight™ Tumor Microtissues can better reflect the cell composition, inter-cellular interaction, tissue structure, and biological characteristics of primary tumors, and are amenable to a variety of biochemical and phenotypic endpoints to help you evaluate and optimize drug potency and efficacy.
Efficacy Testing Applications
Single Drug Testing
Assess single agent efficacy with phenotypic and biochemical endpoints
Combinatorial Drug Testing
Use combinatorial anti-cancer drug efficacy testing to identify synergistic effects
Three‐dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer
Natalie Falkenberg, Ines Höfig, Michael Rosemann, Justine Szumielewski, Sabine Richter, Kenji Schorpp, Kamyar Hadian, Michaela Aubele, Michael J. Atkinson, Nataša Anastasov. Cancer Med. 2016 Apr; 5(4): 703–710. Published online 2016 Jan 14. doi: 10.1002/cam4.630
A 3D-microtissue-based phenotypic screening of radiation resistant tumor cells with synchronized chemotherapeutic treatment
Nataša Anastasov, Ines Höfig, Vanja Radulović, Simon Ströbel, Michael Salomon, Jan Lichtenberg, Ina Rothenaigner, Kamyar Hadian, Jens M. Kelm, Christian Thirion, Michael J. Atkinson
BMC Cancer. 2015; 15: 466. Published online 2015 Jun 10. doi: 10.1186/s12885-015-1481-9
3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained
Michele Zanoni, Filippo Piccinini, Chiara Arienti, Alice Zamagni, Spartaco Santi, Rolando Polico, Alessandro Bevilacqua, Anna Tesei
Sci Rep. 2016; 6: 19103. Published online 2016 Jan 11. doi: 10.1038/srep19103
High-content assays for characterizing the viability and morphology of 3D cancer spheroid cultures.
Sirenko O, Mitlo T, Hesley J, Luke S, Owens W, Cromwell EF. Assay Drug Dev Technol. 2015 Sep;13(7):402-14. doi: 10.1089/adt.2015.655.
3-Dimensional Culture Systems for Anti-Cancer Compound Profiling and High-Throughput Screening Reveal Increases in EGFR Inhibitor-Mediated Cytotoxicity Compared to Monolayer Culture Systems. Amy L. Howes, Robyn D. Richardson, Darren Finlay, Kristiina Vuori. PLoS One. 2014; 9(9): e108283.