Immune Cell Infiltration | InSphero

Immuno-oncology

Immune Cell Infiltration

Evaluate the enhancement of immune cell infiltration following compound treatment

Customized, assay-ready 3D InSight™ Tumor Microtissues provide in vivo-like tissue complexity ideal for evaluating the enhancement of immune cell infiltration following treatment with your compound, or the intrinsic infiltration of engineered immune cells. Immunohistochemical (IHC) characterization of tumor spheroids by functional markers can be used to quantify infiltration over time, with the reproducibility required to ensure assay consistency and confidence in data interpretation.

  • Test in physiologically relevant, immuno-competent co-culture models engineered for ex vivo I-O applications
  • Profile and quantify immune cell infiltration after the addition of your tested drug using a more relevant solid tumor model composed of cell lines or PDX material
  • Study infiltration and effect of CAR-T and CAR-NK in 3D tumor models

Example Data

Monitor immune cell infiltration into tumor microtissues.  Representative IHC of HCC-827 (lung cancer): NhDF (fibroblast) 3D InSight™ Tumor Microtissues co-cultured with activated or non-activated PBMCs. Infiltration of immune cells into the microtissues is observed only when PBMCs are activated. IHC markers: Ki67 (proliferation); Tunel assay (apoptosis); CD3 (T cell marker); CD45 (hematopoietic cell marker); PDL-1 (programmed death ligand 1).

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Brochure: Immuno-Oncology Applications

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Poster: 3D InSight™ Oncology Drug Discovery Platform

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with advanced 3D tumor models.

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