3D InSight™ Platform Add-ons for Assessment of β-cell Function
Through our 3D InSight™ Diabetes Discovery Platform and Platform Add-ons, we offer a comprehensive β-cell function application suite that includes everything you need to quantify insulin secretion from 3D InSight™ Islet Microtissues.
Discover our Platform-add ons
- InSight™ Islet Microtissues are uniform, functionally robust, and long-lived primary islets delivered in an assay-ready, automation-compatible format.
- 3D InSight™ Human Islet Maintenance Medium is optimized for pancreatic islet microtissue culture to support healthy endocrine activity and viability of islet cells in long-term culture.
- 3D InSight™ Krebs-Ringer HEPES Buffer is a physiological solution designed for the assessment of hormone secretion from 3D InSight™ Islet Microtissues.
- ALPCO STELLUX® Chemi Human Insulin ELISA is a highly sensitive enzyme-linked immunosorbent assay (ELISA) with a broad dynamic range ideal for quantifying insulin secretion from 3D InSight™ Islet Microtissues.
Read our application note on assessment of β-cell function in 3D InSight™ Islet Microtissues using the ALPCO STELLUX® Chemi Human Insulin ELISA and learn how you can apply this application suite to take full advantage of two best-in-class systems.
- High sensitivity of STELLUX® ELISA enables accurate quantification of basal insulin secretion from human islet microtissues
- A wide variety of experimental windows for glucose-stimulated insulin secretion from human islet microtissues is supported by the broad dynamic range of STELLUX® ELISA
InSphero Certified – ALPCO STELLUX® Chemi Human Insulin ELISA
The STELLUX® Chemi Human Insulin ELISA measures the concentration of insulin protein products and offers superior performance compared to other similar kits in the market. The assay utilizes a dual-monoclonal antibody sandwich format, tri-level control set, and 96-well microplate comprising removable strips. The assay can measure up to 37 samples in duplicate and can be used in a variety of sample types.
- Broad Dynamic Range (5 – 30,000 pg/mL)
Significantly reduces sample preparation time by eliminating dilution steps and the guesswork involved in determining dilution factors, saving time and money. No guessing, no diluting, no repeating. - Sensitivity
Superior assay sensitivity compared to current competitive products on the market. LOD: <2.0 pg/mL, with a lower limit of quantification of 20 pg/mL. - One Assay to Fit All Sample Types
The assay works with human serum, heparin plasma and tissue culture supernatants. - Lot Validation Package
TLot validation includes functional sensitivity, inter- and intra-assay precision, accuracy, LLOQ , and ULOQ.
Our Diabetes Platform development team rigorously tested various commercially available insulin ELISAs on the market, and ALPCO’s STELLUX® ELISA technology either met or exceeded all our criteria for certification.
Certified Applications
| Approved for assessment of | Available as |
|---|---|
| Insulin secretion | MM | AS | TP |
| Insulin content | MM | AS | TP |
| Islet viability | MM | AS | TP |
MM = Microtissue Model
AS = Assay Service
TP = Technical Protocol
Order Information
| Catalog # | Description |
|---|---|
| Microtissues | |
| MT-04-002-01 | 3D InSight™ Human Islet Microtissues, 96 well plate (1/well) |
| Media and Buffers* | |
| CS-07-005-01 | Human Islet Maintenance Medium, 500 mL |
| CS-07-005-02 | Human Islet Maintenance Medium, 250 mL |
| CS-07-050-03 | Human Islet Assay Medium, 125 mL |
| CS-07-051-01 | Krebs-Ringer HEPES Buffer, 500 mL |
| Kits* | |
| 80-INSHU-CH01 | ALPCO STELLUX® Chemi Human Insulin ELISA, 1 96 well plate |
| 80-INSHU-CH10 | ALPCO STELLUX® Chemi Human Insulin ELISA, 10 96 well plate |
* Available only when purchased with 3D InSight™ Microtissues
