3D InSight™ Liver Microtissues, 3D Liver Models | InSphero

3D InSight™ Liver Microtissues are standardized 3D liver models, produced using primary hepatocytes and non-parenchymal liver cells. These advanced liver models accurately reflect the structure and biology of in vivo liver, delivering greater confidence in decision-making based on in vitro cell-based assays for liver injury and drug efficacy testing.

Explore key mechanisms of healthy and diseased liver function

Our liver microtissues are comprised of primary human or animal hepatocytes and non-parenchymal liver cell types, such as Kupffer cells and liver endothelial cells, resulting in native-tissue-like functionality. They exhibit albumin secretion, polarized cellular organization, robust cytochrome P450 activity, high mitochondrial function, and bile-acid transport to the bile canaliculi, and into the surrounding media. This long-lived model improves sensitivity for DILI prediction by enabling long-term drug exposure and by including a physiologically relevant ratio of primary hepatocytes to non-parenchymal cells.

Improve sensitivity for DILI prediction by employing long-term drug exposure

Tested with 110 compounds, 3D InSight™ Human Liver Microtissues showed 2x higher sensitivity than 2D PHH for detection of DILI compounds. Read the Arch Toxicol Paper

Ensure reproducibility and continuity in longitudinal and multi-site studies

Order standardized, pre-qualified liver models, certified for liver toxicology applications, such as DILI prediction, that are available for delivery direct to your lab when you’re ready to start experiments. Check our production schedule

Human and Animal-derived Liver Microtissues

Human

Primary human-derived liver microtissues available biweekly.

Monkey

Primary cynomolgus-derived liver microtissues available on demand.

Dog (early access)

Primary beagle-derived liver microtissues available on demand.

Rat

Primary Sprague-Dawley-derived liver microtissues available on demand.

“Making primary hepatocyte-derived liver microtissues containing the appropriate number of non-parenchymal cells is complicated. In a European consortium, which performed a ring trial with 3D liver models, multiple labs failed to produce liver models themselves despite all using the same protocol and donor cells. When using InSphero’s assay-ready 3D InSight™ Microtissues, you avoid high upfront costs and frustrations, so you can focus on the most important part: generating reproducible data with a robust 3D model you can rely on.”

RADINA KOSTADINOVA, PHD

3D InSight™ Liver Microtissue Product Manager

Debating whether to make or buy your liver microtissues? Read this first.

InSphero 3D InSight™ Liver Microtissues are next-generation 3D models for DILI and investigative toxicity testing.  Using the 3D Select™ Process, we ensure only the most viable hepatocytes incorporate into our microtissues, improving viability and organotypic morphology.  When compared head-to-head to conventional production methods, there is no comparison. See the results in this white paper:

Perfecting cellular self-assembly: Prerequisites for industrial-scale production of 3D microtissues for drug discovery and development.

Request Additional Resources

Brochure: Solutions for Toxicology

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Publication: Proctor et al. (Arch Toxicol, 2017)

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Poster: Drug Safety Assessment with 3D InSight™ Liver Microtissues

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Find out how 3D InSight™ Liver Microtissues can provide greater

confidence in safety testing of your drugs.

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