Fibrosis Model | InSphero

Test efficacy of anti-fibrotic drugs and explore mechanisms of fibrosis induction

The InSphero 3D InSight™ Human Liver Fibrosis Model consists of an advanced microtissue co-culture of healthy primary human liver cells combined with specially formulated media and a TGF-β1 solution that induces the formation of fibrotic scarring. This model is designed for in vitro screening of anti-fibrotic drug efficacy and for studying mechanisms of fibrosis induction. It is engineered to incorporate all relevant human liver cells needed to recapitulate the fibrotic disease state, including hepatocytes, Kupffer cells, hepatic stellate cells, and liver endothelial cells. This powerful liver disease model has been rigorously characterized under basal and induced fibrotic conditions using histological techniques (IHC and IF), gene-expression analysis (qPCR) and secreted biomarker (ELISA) analysis.

3D human preclinical spheroid model for liver fibrosis drug discovery

The 3D InSight™ Human Liver Fibrosis Model enables the study of liver fibrosis induction and inhibition of disease progression. The screening-compatible 3D microtissue format provides maximal endpoint compatibility.

Model Specifications

  • Human liver model produced with PHH from pooled male/female donors in co-culture with Kupffer cells, hepatic stellate cells, and LECs
  • Guaranteed 21 days viability (before disease induction)
  • 96-well format, 1 microtissue per well

Platform Components*

Catalog # Description
MT-02-302-05 3D InSight™ Human Liver Disease Microtissue (healthy primary human hepatocytes, co-culture with Kupffer cells, hepatic stellate cells, liver endothelial cells), 96x
KT-04-400-01 Liver Fibrosis Kit
CS-07-001-01* Maintenance Medium – TOX, 500 mL

*3D InSight™ Liver Disease Discovery Platform Bundles are customized based on specific partner requirements. Not available separately.

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Brochure: Human Liver Fibrosis Model


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