3D InSight™ Human Liver Microtissues, Human Liver Models| InSphero

Closely approximate the human response to compound exposure

3D InSight™ Human Liver Microtissues are a more sensitive 3D co-culture model compared to 2D PHH for DILI prediction, that combines multi-donor diversity with structural and functional robustness. Upon request, microtissues from individual donor hepatocyte lots are also available.

  • Reflect the average response of a population with a model incorporating hepatocytes from 5 male and 5 female donors
  • Achieve the highest biological relevance in an in vitro model with primary hepatocyte, Kupffer cell, and liver endothelial cell (LEC) co-cultures
  • Test long-term effects of drug exposure in an organotypic, functional liver model

Characterization Data

Human Liver Microtissue Specifications

  • PHH from pooled male/female donors
  • Co-culture with Kupffer cells and LECs
  • Guaranteed 28 days viability
  • 96-well format, 1 microtissue per well
  • 384-well format coming soon
  • Biweekly production

Order Information

Catalog #Description
Microtissues
MT-02-302-043D InSight™ Human Liver Microtissues (multi-donor hepatocytes, co-culture with Kupffer cells and LECs), 96x
MT-02-302-013D InSight™ Human Liver Microtissues (multi-donor hepatocytes, monoculture), 96x
MT-02-002-043D InSight™ Human Liver Microtissues (single-donor hepatocytes, co-culture with Kupffer cells and LECs), 96x
MT-02-002-013D InSight™ Human Liver Microtissues (single-donor hepatocytes, monoculture), 96x
Kits
KT-02-121-143D InSight™ DILI Evaluation Kit (6 plates of 96x Multi-donor microtissues, media, ATP assay, and reference compounds)
AK-01-001-013D InSight™ Cytotoxicity Assay Kit for up to 4x 96-well plates
Media*
CS-07-001-01Maintenance Medium – TOX*
(for tox testing), 500 mL
CS-07-001a-01Maintenance Medium – AF*
(for basic cultivation), 500 mL
CS-07-001b-01Maintenance Medium – INF*
(for inflammatory tox testing), 500 mL

* only available when purchased with 3D InSight™ Microtissues

Request Additional Resources

Brochure: Human Liver Microtissues

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Publication: Proctor et al. (Arch Toxicol, 2017)

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Poster: Multi-donor Human Liver Microtissues

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Find out how 3D InSight™ Liver Microtissues can provide greater

confidence in safety testing of your drugs.

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