Cytotoxicity Assay Kit | InSphero

Monitor cell viability in 3D InSight™ microtissues with Promega technology

The 3D InSight™ Cytotoxicity Assay Kit is a homogeneous biochemical assay for measuring total cellular ATP as cell viability endpoint. Certified for use with InSphero 3D InSight™ microtissues and culture media, this kit incorporates proprietary luminescent technology from Promega, enabling you to:

  • Quantify metabolically active cells using a rapid, homogeneous luminescent ATP assay
  • Increase assay reproducibility with an efficient lysis agent proven compatible with 3D InSight™ microtissues and media
  • Simplify ordering by getting everything you need (microtissues, media, and assay kit) for cytotoxicity testing from one source

Kit Contents

Each 3D InSight™ Cytotoxicity Assay Kit includes the following components, suitable for quantifying ATP in up to 4 x 96-well plates of 3D InSight™ microtissues (including controls):

  • CellTiter-Glo® 2.0 Reagent, 10 mL
  • 10 µM Adenosine-5’-triphosphate (ATP) standard, 750 µL
  • Kit product manual with protocol optimized for 3D InSight™ microtissues

Order Information

Catalog # Description
AK-01-001-01 3D InSight™ Cytotoxicity Assay Kit

Compatible Products

Note: InSphero recommends use of the Promega CellTiter-Glo® 3D Cell Viability Assay for determining ATP content in larger spheroids, such as those generated with actively proliferating tumor cell lines, as larger spheroids may exceed the lytic capacity of the assay reagent provided in this kit.

Request Additional Resources

Brochure: Cytotoxicity Assay Kit


Publication: Proctor et al. (Arch Toxicol, 2017)


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