3D InSight™ Custom Tumor Microtissues, Custom tumor Models | InSphero

Standardized tumor model development using the InSphero 3D Select™ Process

We can work with you to develop a custom tumor model to fit your project using our 3D Select™ Process and patented InSphero scaffold-free assay platforms. Select or provide a tumor cell line or PDX sample, and we’ll optimize conditions for microtissue formation to develop monoculture or co-culture (with fibroblasts) models to fit your needs. Once established, the model can be used in 3D InSight™ Oncology Services or delivered assay-ready for use in your lab.

Custom Tumor Model Specifications

Service Description

    • Feasibility and optimization of spheroid formation
    • Size-based growth kinetics over 10 days in culture
    • ATP after tissue formation and 10 days in culture
    • Histology at day 4 and 10 (H&E plus 3 markers)
    • Full written report and consultation

Model specifications

  • Viability model-dependent
  • 96-well format, 1 microtissue per well
  • Produced on demand; production schedule dependent on cell line or PDX material

Order Information

Supply your own cell line, or select from one of the many established tumor cell lines available for custom model development.

Tumor Type Cell Line Fluorescence 3D Monoculture
Breast MCF-7
TD-47D*
MDA-MB-231
MDA-MB-361*
BT474

RFP
RFP
RFP
no
yes
no
yes
yes
Colorectal HT-29
DLD-1*
LS174T*
HCT-116*
LoVo


GFP
GFP
yes
yes
yes
yes
yes
Stomach NCI-N87* GFP yes
Lung A549*
H292
NCI-H460
NCI-H2170
NCI-H1975
NCC-827
GFP




yes
yes
yes
yes
yes
yes
Kidney A498*
786-O*
COLO-205


yes
yes
no
Prostate 22RV1
DU-145*
LnCap
PC-3



yes
yes
yes
no
Brain SNB-19
LN-18

GFP
yes
yes
Pancreas PANC-1* GFP no
Ovarian A2780
SKOV-3
HEY*


GFP
yes
no
yes
Liver HepG2* yes

Request Additional Resources

Brochure: Tumor Microtissues

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Poster: 3D InSight™ Oncology Platform

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