3D InSight™ Antibody Penetration Assay Service | InSphero

Assess penetration of biologicals and ADCs in 3D InSight™ Tumor Microtissues

Penetration and distribution of biologics can depend on a variety of factors, such as tissue density, antibody affinity, antigen density per cell and the physico-chemical properties of the biologic. The spherical, 3D structure and more native ECM and biology of 3D InSight™ Tumor Microtissues makes them ideal for assessing penetration of antibodies and engineered antibody fragments in vitro.

  • Test kinetics of antibody/ADC penetration and distribution in biomimetic 3D tumor models with native-like ECM and tissue density
  • Utilize immunohistochemical detection techniques to visualize the distribution of your biologic in the tumor tissue
  • Receive model suitability assessment, detection method detail, and penetration kinetic report
  • Optimize biological targeting and efficacy by comparing multiple constructs (e.g., full-length IgG, IgG Fc, sFv, bi-specific)

Characterization Data

Service at a Glance

Staged as three phases: Feasibility, Optimization, and Assessment. Download our brochure for more details.

Service Report includes

  • Feasibility report for Phase 1 assessment
  • Method description and imaging results for immunofluorescence (IF) detection of target antibody
  • Penetration kinetic based on IF for target antibody and control (anti-beta integrin)
Catalog #Description
SP-01-044-013D InSight™ Antibody Penetration Assay
(Phase 1 – Model characterization)
3D InSight™ Antibody Penetration Assay
(Phase 2 – Biologic detection)
3D InSight™ Antibody Penetration Assay
(Phase 3 – Kinetic penetration assay)

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Brochure: Oncology Services

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Brochure: Tumor Microtissues

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