Efficient, animal-free in vitro embryotoxicity screening for DART
The embryonic stem cell test (EST) is a powerful method for developmental and reproductive toxicity (DART) testing, used to assess the effects of any new compound on cell differentiation and viability. Introduced in 1997, the EST quickly became recognized as the best available method for embryotoxicity testing that avoids the use of animals. However, the classical EST protocol (ICCVAM/ECVAM INVITTOX No. 113) is laborious and complex, which has significantly hampered its adoption in industry. We incorporate InSphero microtissue production technologies into the ECVAM protocol to improve EST efficiency and reproducibility, eliminating the practical issues with the classical EST protocol.
- Use a secure protocol that parallels ECVAM Invittox 113
- Assess embryotoxicity more cost-effectively using an efficient embryoid body (EB)-based EST
- Consume less of your valuable compounds in an in vitro assay format that doesn’t require the use of laboratory animals
Service Description
Service at a Glance
Download our brochure for more details on the standard EST protocol. The service includes:
- A web-based presentation and discussion
- Embryotoxic classification with linear discriminants according to ECVAM INVITTOX protocol No. 113
- A summary report including IC50 data for 3T3 and ESD3 cells (n=6) and ID50 data for ESD3 cells (n=24)
| Catalog # | Description |
|---|---|
| SP-03-001-00 | SP-03-001-00 3D InSight™ Embryonic Stem Cell Test (EST) Pack – 4 compounds |
| SP-03-002-00 | SP-03-002-00 3D InSight™ Embryonic Stem Cell Test (EST) Pack – 8 compounds |
| SP-03-003-00 | SP-03-003-00 3D InSight™ Embryonic Stem Cell Test (EST) Pack – 16 compounds |
