Antibody Penetration Services | InSphero

Assess antibody penetration and engineered antibody fragments in vitro

Antidoby penetration and distribution of biologics can depend on a variety of factors, such as tissue density, antibody affinity, antigen density per cell, and physicochemical properties.

3D structure, native ECM, and biology of 3D InSight™ Tumor Organoids make them ideal for assessing penetration of antibodies and engineered antibody fragments in vitro.

3D InSight™ Tumor Organoids Standard Protocol

  • Phase 1: Model Characterization assessment of model feasibility for antibody penetration assay using beta integrin antibody
  • Phase 2: Biologic Detection optimization of biologics protocol of interest by immunofluorescence (IF)
  • Phase 3: Kinetic Penetration Assay assessment of antibody penetration over 3-time points

Antibody Penetration Services at a Glance

Download our Oncology Services brochure for more details on our antibody penetration services.

Service report includes

  • Feasibility report for Phase 1 assessment
  • Method description and imaging results for IF detection of target antibody
  • Penetration kinetic based on IF for target antibody and control (anti-beta integrin)
Catalog # Description
SP-01-044-02 3D InSight™Antibody Penetration Assay
(1 model, 1 antibody, 1 concentration)

Request Additional Resources about our Antibody Penetration Services

Brochure: Oncology Services


Poster: Oncology Platform


Brochure: Immuno-Oncology Services


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