3D InSight™ Antibody Penetration Assay Service | InSphero

Assess penetration of antibodies and engineered antibody fragments in vitro

Penetration and distribution of biologics can depend on a variety of factors, such as tissue density, antibody affinity, antigen density per cell and physico-chemical properties. The spherical, 3D structure and more native ECM and biology of 3D InSight™ Tumor Microtissues makes them ideal for assessing penetration of antibodies and engineered antibody fragments in vitro.

Standard Protocol

  • Phase 1: Model Characterization assessment of model feasibility for antibody penetration assay using beta integrin antibody
  • Phase 2: Biologic Detection optimization of detection protocol for biologics of interest by immunofluorescence (IF)
  • Phase 3: Kinetic Penetration Assay assessment of antibody penetration kinetics over 3 time points

Service at a Glance

Download our Oncology Services brochure for more details.

Service report includes

  • Feasibility report for Phase 1 assessment
  • Method description and imaging results for IF detection of target antibody
  • Penetration kinetic based on IF for target antibody and control (anti-beta integrin)
Catalog # Description
SP-01-044-02 3D InSight™Antibody Penetration Assay
(1 model, 1 antibody, 1 concentration)

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Brochure: Oncology Services

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Poster: Oncology Platform

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Brochure: Immuno-Oncology Services

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