Assess antibody penetration and engineered antibody fragments in vitro
Antidoby penetration and distribution of biologics can depend on a variety of factors, such as tissue density, antibody affinity, antigen density per cell, and physicochemical properties.
3D structure, native ECM, and biology of 3D InSight™ Tumor Organoids make them ideal for assessing penetration of antibodies and engineered antibody fragments in vitro.
3D InSight™ Tumor Organoids Standard Protocol
- Phase 1: Model Characterization assessment of model feasibility for antibody penetration assay using beta integrin antibody
- Phase 2: Biologic Detection optimization of biologics protocol of interest by immunofluorescence (IF)
- Phase 3: Kinetic Penetration Assay assessment of antibody penetration over 3-time points
Antibody Penetration Services at a Glance
Download our Oncology Services brochure for more details on our antibody penetration services.
Service report includes
- Feasibility report for Phase 1 assessment
- Method description and imaging results for IF detection of target antibody
- Penetration kinetic based on IF for target antibody and control (anti-beta integrin)
| Catalog # | Description |
|---|---|
| SP-01-044-02 | 3D InSight™Antibody Penetration Assay (1 model, 1 antibody, 1 concentration) |
