3D InSight™ Cancer Drug Efficacy Testing | InSphero

Biochemical and phenotypic assessment of drug potency and efficacy testing

Drug efficacy testing for your compounds or biologics of interest to assess their potency (EC50) and efficacy (maximum response) on tumor organoids using 3D-optimized biochemical (ATP) and phenotypic (size) endpoints.

Efficacy testing service protocol

  • Choose a 3D InSight™ Tumor model and supply at least 2 test compounds
  • Choose controls (e.g., Staurosporine, MEK162, Ipatasertib, Sorafenib/Sunitinib, DMSO, etc.)
  • Produce a 9-point dose response in quadruplicate
  • Perform an 11-day efficacy testing assay with dosing at days 0, 4, and 7

Efficacy Testing Characterization Data

Slide Evaluate anti-tumor efficacy in physiologically relevant 3D models. Receive ATP-based dose-response curve and dose-dependent size-based growth kinetics. Slide Leverage non-disruptive, continuous size assessment to monitor tumor response over time, preserving samples for optional customized endpoints. Potency and efficacy of the MEK inhibitor Binimetinib (MEK-162) in HepG2 spheroids.

Efficacy Testing at a Glance

Download our brochure for details on the standard protocol.

Drug Efficacy Testing Service Report includes

  • ATP-based dose-response curve at day 11
  • Size-based dose-response curve and growth kinetics over time
  • Efficacy testing (IC50) values
Catalog # Description
SP-01-041-02 3D InSight™ Efficacy Testing Service
(2 or more compounds, 2 controls, 1 model)

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