3D InSight™ | 3D In Vitro Liver Toxicology
Your proven end-to-end 3D in vitro liver safety solution portfolio - from reliable hazard identification to insights into translational and clinical toxicology
Industry-ready 3D In Vitro Liver Toxicology Solutions
InSphero’s 3D InSight™ Liver Safety solutions deliver precise and predictive 3D in vitro liver models for liver toxicity assessment since 2013. Our 3D in vitro liver toxicology technology accurately models the intricate architecture and functions of the human liver with a physiologically relevant microenvironment to enable better assessments of drug metabolism and toxicological responses. By incorporating our 3D InSight™ Liver Safety solutions into your workflow, you will gain valuable insights into:
- Predictive toxicology - Drug-Induced Liver Injury (DILI)
- Mechanistic/Investigative toxicology
- Translational toxicology - Animal to human translational toxicology
3D InSight™ Liver Microtissues, our proprietary spherical liver models, are composed of primary hepatocytes and non-parenchymal liver cells and are available in Akura™ 96 & 384 spheroid microplates.
Variants of the 3D InSight™ liver microtissues are available for both human and preclinical species. These models allow for the measurement of a wide range of hepatic biomarkers, including but not limited to:
- ATP
- ALT leakage
- Cytochrome P450 activity
- Albumin secretion
- Cytokine secretion
- Bile acid secretion
- Glycogen,
- Triglycerides, as well as data from transcriptomics, proteomics, and lipidomics
The physiological relevance of the 3D InSight™ liver microtissues, broad range of specialized in vitro assays available, combined with the scalability, robustness, and reproducibility of InSphero’s technology makes of InSphero’s 3D in vitro liver toxicology solutions an industry-ready solution for both the screening of large set of compounds early in the drug development process and the in depth and complex evaluation of a small number of selected drug candidates before first in human studies.
Discover the application of InSphero's Liver Safety Solutions in peer-reviewed papers:
Fäs, L., Chen M., Tong W. et al. Physiological liver microtissue 384-well microplate system for preclinical hepatotoxicity assessment of therapeutic small molecule drugs. Toxicological Sciences 203, 79–87 (2025)
Shinozawa, T., Miyamoto, K. et al. TAK-994 Mechanistic Investigation into Drug-Induced Liver Injury. Toxicological Sciences, January 2025
Bajaj, P., Brennan, R. J. et al. Transcriptomic Analysis in Liver Spheroids Identifies a Dog-Specific Mechanism of Hepatotoxicity for Amcenestrant. January 2025
Proctor, W.R., Foster, A.J., Vogt, J. et al. Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury. Arch Toxicol 91, 2849–2863 (2017)
Messner, S., Agarkova, I., Moritz, W. et al. Multi-cell type human liver microtissues for hepatotoxicity testing. Arch Toxicol 87, 209–213 (2013)
Why 3D In Vitro Liver Toxicology with InSphero?
InSphero’s 3D In Vitro Liver Safety Platform has been developed with the needs of the safety toxicologists in mind: 3D in vitro human liver models with high biological and physiological relevance, short turn-around times, capable of handling larger compound sets when needed, and high reproducibility decision-enabling datasets.
Market-leading 3D in vitro biology you can rely on
Trusted by pharmaceutical companies worldwide
Comprehensive 3D In Vitro Liver Toxicology platform for efficient and fast projects
Our Approach to 3D In Vitro Liver Toxicology
Discover mitigation routes by understanding the mechanism of action of in vitro liver hepatotoxicity through transcriptomics and causality assays.
