Discover how InSphero’s 3D InSight™ Human Neural Microtissues and MaxWell Biosystems’ MaxTwo Multiwell HD-MEA System combine human-relevant 3D neural biology with high-content functional electrophysiology. This application note demonstrates scalable, label-free profiling of spontaneous and compound-modulated neuronal network activity, capturing distinct responses to excitatory and inhibitory reference compounds. Advanced network and burst-shape analyses further reveal subtle, mechanism-dependent differences in network dynamics, providing deeper functional insight for compound screening, safety assessment, and neuropharmacology.