RNA Sequencing in Spheroids
Versatile solution to study transcriptomes in physiologically relevant 3D in vitro spheroid models
Introduction to RNA Sequencing in Spheroids
RNA sequencing (RNA-Seq) is a subfamily of Next Generation Sequencing (NGS) techniques that reveal the presence and quantity of various RNA molecules in a sample, providing insights into gene expression and regulation. It is used to study the transcriptome, identify gene expression patterns, and contribute to our understanding of cellular functions and disease mechanisms.
RNA sequencing in spheroids is a cutting-edge technique used to study gene expression in three-dimensional cellular structures that closely mimic the in vivo environment of tissues. Unlike traditional 2D cultures, 3D spheroids provide a more physiologically relevant context, capturing cell-cell and cell-matrix interactions.
RNA-Seq involves extracting RNA from these spheroids and sequencing it to quantify gene expression levels. This approach enables researchers to analyze the transcriptome dynamics of cells in a more natural state, facilitating insights into complex biological processes, disease mechanisms, and potential therapeutic targets.
Why RNA Sequencing in Spheroids with InSphero?
We provide relevant biological signals at scale! Our all-in-one RNA Sequencing solution, tailored for 3D Cell Culture models, ensures that you capture and interpret relevant biological signals with unparalleled accuracy and scalability. This empowers your research with data that truly reflects complex physiological conditions.
Worry-free Study Execution
Insightful gene expression signatures are guaranteed by optimized RNA-Seq workflows utilizing physiology-relevant 3D InSight™ Microtissues and compatible high-throughput Akura™ Microplate Technology.
RNA-Seq Readout for Single 3D Spheroids
Reliable gene expression profiling from single spheroids or pools of three microtissues accomplished through RNA-Seq methodologies attuned to low-input material.
Tailored RNA-Seq Analysis
Customer and project-specific analysis of gene expression results enabled by a dedicated and modularized RNA-Seq analysis pipeline.
Intuitive Interpretation of RNA-Seq Results
A comprehensive report dashboard facilitates effortless interpretation of RNA-seq results, ensuring that complex data is easily understandable and actionable.
RNA Sequencing in Spheroids for Scalable, Reliable and Reproducible Transcriptomic Discoveries
Obtain meaningful transcriptomic signatures from well-established RNA-Seq workflows
Experience worry-free study execution and obtain relevant biological signals at scale with our optimized workflows for RNA Sequencing in spheroids. Seamless project planning and study design are driven by InSphero’s experienced team, leveraging extensive internal know-how. Utilizing our robust physiology relevant 3D InSight™ Liver and Islet Microtissues ensures insightful gene expression signatures. Our well-established processes and high-throughput Akura™ Microplate Technology minimize technical biases, enabling you to compare numerous intervention groups with trustworthy statistical relevance.
Robust transcriptomic profiling is accomplished with RNA-Seq assays optimized for spheroids
Achieve reliable gene expression profiling from single spheroids or pools of three microtissues with InSphero's RNA-Seq methodologies, optimized for low-input material. Our robust RNA Sequencing in 3D assays, tailored specifically for spheroids, deliver unparalleled biological resolution. InSphero offers a highly flexible and comprehensive RNA-Seq service, enabling precise answers to your project-specific questions. Utilizing three advanced methodologies - low-input bulk RNA-Seq, whole transcriptome TempO-Seq assay, and DRUG-Seq - we provide high-throughput gene expression profiling for projects of all sizes and budgets, ensuring insightful and cost-effective results.
Project-customised gene expression assessment via advanced and modularized
RNA-Seq analysis pipeline
High-throughput experiments with RNA sequencing in spheroids generate complex, multi-dimensional data that requires advanced statistical methods and sophisticated bioinformatics software for accurate processing. InSphero's RNA-Seq analysis pipeline offers automated, highly structured, and reliable RNA-Seq data processing. Powered by the R programming language and leveraging a wide range of cutting-edge R libraries, our pipeline is divided into functional blocks to streamline each stage of the analysis. To accommodate various budgets and project needs, we offer a range of RNA-Seq analysis packages, each designed to provide different levels of depth and customization.
Effortless interpretation of your RNA sequencing results facilitated by an interactive RNA-Seq report dashboard
Unlock the full potential of your gene expression data with InSphero’s interactive RNA-Seq report dashboard, designed to make data interpretation both intuitive and insightful. Our advanced RNA-Seq analysis pipeline produces a comprehensive array of numeric outputs and sophisticated visualizations, ensuring that even the most intricate gene expression patterns are easily understood. Each table and graphic in our interactive dashboard offer immediate clarity, transforming complex next-generation sequencing (NGS) results into actionable insights. With user-friendly features and modern data presentation, our dashboard enhances your experience by providing seamless access to vital information and allowing for efficient exploration of your data. Enjoy the benefits of streamlined analysis and gain a deeper understanding with minimal effort, empowering you to make informed decisions quickly and effectively.
RNA Sequencing in Spheroids: Choose the Right Analysis Package and Report Dashboard for Your Research
Explore the portfolio of RNA-Seq Analysis Packages and associated Report Dashboards to unlock the full potential of RNA Sequencing in spheroids.
Related Services
Technologies for RNA Sequencing in Spheroids
Low-input RNA-Seq
Low-input RNA-Seq is designed for situations where the RNA quantity is limited, making it ideal for small 3D spheroid models. It focuses on sequencing mRNAs, capturing a broad range of poly-A transcripts with high sensitivity for detecting differential gene expression. This method is species-agnostic making it suitable for various research models. It is a versatile option for small to mid-size projects requiring unrestricted and in-depth gene expression analysis from minimal input material.
Whole Transcriptome TempO-Seq
Whole Transcriptome TempO-Seq assay is a sequencing method that targets a pre-selected set of ~19-22K mRNAs. However, it is limited to human, rat, and mouse species. Its cost-efficiency and capability for high-throughput make it a practical choice for mid- to large-size projects with limited budgets aiming at robust and sensitive transcriptome-wide gene expression profiling.
Organoid DRUG-Seq
Organoid DRUG-Seq is optimized for high-throughput screening of drug responses in 3D spheroids, allowing researchers to efficiently profile gene expression changes in response to different treatments. It is also species-agnostic, making it versatile for various drug testing scenarios. Its scalability and affordability make it an excellent choice for large-scale projects aiming at screening the most prominent gene expression changes in a cost-effective fashion.