Pancreatic islet perifusion assays have long been used to measure dynamic hormone release from islet cells in response to specific stimuli, however implementing a reliable, highly reproducible, yet simple method to assess human islet function remains challenging.
In this new webinar, InSphero Vice President of Islet Biology, Dr. Burcak Yesilag teams up with Dr. Patrick Misun of the ETH Zürich Department of Biosystems Science and Engineering, to discuss how dynamic insulin release can be studied in vitro using a novel microfluidic perifusion system. They present results of their collaborative study, recently published in Advanced BioSystems, and offer their vision for the future of preclinical islet research.
You will learn:
- Limitations of pancreatic islet perifusion assays that rely on pooling multiple islets per experimental condition
- Model requirements for reliably measuring secretion dynamics of a single human islet in vitro, using our 3D InSightâ„¢ Diabetes Platform for islet research as an example
- How the newly engineered microfluidic hanging-drop-based perifusion platform was used to study dynamic insulin release from single islets at high temporal resolution