Organoids are complex, self-organizing, three-dimensional (3D) in vitro cell culture models. They derive from stem cells, resemble their in vivo counterpart and recapitulate various functions of the native organ. The use of organoids has major advantages including easy access to human biology, genetic stability, significant expansion potential and their possible cryopreservation in biobanks. These near-physiological models are increasingly popular in pre-clinical research. Since the development of the first mouse intestinal organoids in 2009, multiple organoid models have been established. However, these organoid cultures heavily rely on the use of solid extracellular matrix. This classical method introduces a high level of heterogeneity in the cultures, both in terms of size and shape. It also complicates assay development as organoids are positioned randomly in the ECM drops. Gri3D® was specifically designed to overcome these challenges. Gri3D® is a ready-to-use platform for high-throughput and reproducible organoid culture. The resulting organoids are homogeneously sized and are confined within hydrogel microwells, thus facilitating their imaging. Gri3D® provides an ideal tool to standardize organoid cultures.