3D InSight™ Human Beta Cell Proliferation Service
Combining our standardized human islet microtissues with 3D high-content imaging and automated 3D image analysis for the in vitro assessment of pancreatic β-cell proliferation with unmatched predictive power
Isolated donor islets, traditionally used for the study of human β-cell proliferation, suffer from rapidly deteriorating viability and function ex vivo in their intact form, and once dispersed, their β-cells dedifferentiate and hyperproliferate in culture. In contrast, islet microtissues, produced by reaggregation of gently dispersed human islet cells, represent a uniform, functionally robust and long-lived in vitro islet model that preserve native-like endocrine composition and cell-to-cell interactions critical for physiologically relevant proliferative responses. Uniquely, long-term stable functionality and viability of islet microtissues enables in vitro assessment of changes in β-cell and islet mass in addition to proliferation.
Our automated 3D staining, high-content imaging and image analysis platform was developed to utilize human islet microtissues for the evaluation of β-cell and non-β-cell proliferation, fraction and mass, and for the correlation of these descriptive data with functional endpoints for high-throughput study of novel proliferative agents.
Why Study β-cell Proliferation with InSphero?
Retain physiological relevance with increased throughput
Achieve the physiological relevance of a 3D primary human islet model with the throughput of a 2D assay platform
Expand assay windows
Profit from unbiased, automated image analysis
Obtain reliable and unbiased data in record time through our streamlined, automated and 3D optimized image analysis workflows
Correlate your proliferation data with islet function
Assess potential impact on islet function in parallel to proliferation
Our Process
Plan with diligence
We start by selecting the right donor, treatment duration, dosing scheme and stress model suitable for your goals
Execute with precision
Next, islet microtissues treated with your compound or modified for the expression of your target, are subjected to our streamlined 3D-optimized assays and automated analysis pipelines
Conclude with confidence
We help you achieve reproducible and high-quality results that will drive meaningful conclusions.