3D InSight™ Human Beta Cell Proliferation Service
Combining our standardized human islet microtissues with 3D high-content imaging and automated 3D image analysis for the in vitro assessment of pancreatic β-cell proliferation with unmatched predictive power
Isolated donor islets, traditionally used for the study of human β-cell proliferation, suffer from rapidly deteriorating viability and function ex vivo in their intact form, and once dispersed, their β-cells dedifferentiate and hyperproliferate in culture. In contrast, islet microtissues, produced by reaggregation of gently dispersed human islet cells, represent a uniform, functionally robust and long-lived in vitro islet model that preserve native-like endocrine composition and cell-to-cell interactions critical for physiologically relevant proliferative responses. Uniquely, long-term stable functionality and viability of islet microtissues enables in vitro assessment of changes in β-cell and islet mass in addition to proliferation.
Our automated 3D staining, high-content imaging and image analysis platform was developed to utilize human islet microtissues for the evaluation of β-cell and non-β-cell proliferation, fraction and mass, and for the correlation of these descriptive data with functional endpoints for high-throughput study of novel proliferative agents.
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