The Akura™ Twin Organ-on-Chip Platform is designed for organ-organ cross-talk in the presence of immune cells with robust handling as well as reliable culturing and analysis.
Address present challenges in drug development:
Experience an innovative and intuitive well architecture:
Rely on universal plate standards:
Access a wide range of readout methods:
Bring Akura™ Twin Organ-on-Chip Platform into your lab by acquiring our starter kit offering. It’s everything you need to immediately begin investigating complex biological questions in your research:
Controls gravity-based flow in incubator with intuitive built-in user interface
Pack of 5 plates for up to 192 experimental conditions per plate
Simple handling tool providing gravity-based flow while performing medium exchange under the hood.
For 3D spheroid aggregation before transferring into Akura™ Twin or for collection after experiments and downstream analysis
Learn about concept, design and characteristics of the Akura™ Twin Platform.
Personal support and consulting to set up the first experiments with InSphero’s scientific experts.
Allows for discounted Refills & Voucher (2000 CHF) for InSphero products (valid 6 months)
A detailed experimental protocol for the Akura™ Twin Organ-on-Chip Platform is provided in the product manual. Below are answers to some frequently asked questions to help you get started.
The Akura™ Twin Organ-on-Chip Platform is designed to support various scaffold-free 3D cell culture models, including organoids, spheroids, and microtissues. The ULA coating of the wells and microchannels helps preserve model integrity and minimizes cell adhesion during long-term culture. For optimal results, we recommend that your 3D model meet the following criteria:
We have successfully used both stimulated and unstimulated PBMCs (peripheral blood mononuclear cells). In principle, any cell type that survives in suspension as single cells can be used in the platform. We recommend optimizing cell concentrations for your specific application.
Flow in the Akura™ Twin Plates is generated by a gravity-driven perfusion system. When the plate is placed on the Akura™ Programmable All-in-One Tilter inside a standard laboratory incubator, it tilts back and forth around its short axis. This motion induces bidirectional flow through the connecting wells. Tilting angles of 20–40° are typically used. No tubing or pumps are required.
Yes, we highly recommend using the Akura™ Programmable All-in-One Tilter to enable gravity-based flow in the Akura™ Twin Organ-on-Chip Platform.
The tilter is designed for automated flow control and fits inside a standard laboratory incubator. It provides programmable reciprocal motion with adjustable tilt angles (0° to ±85°), pause durations, and interval settings, controlled via an integrated microprocessor.
These settings allow precise control of flow parameters such as shear stress, velocity, and flow rate. Conventional cell culture rockers typically lack programmable pause functions in tilted positions, limiting flow control.
The Akura™ Tilter is required to generate gravity-based flow between the connected wells. Without tilting, no exchange of soluble factors or cells occurs.
These channels are aligned with the axis of rotation and experience no effective tilting. As a result, they can be used as “no-flow” controls for comparison.
The plate body is made of polystyrene, and the bottom membrane is made of cyclo-olefin polymer (COP) with a thickness of 188 µm. COP is highly transparent (similar to glass) and offers 92% light transmission in the 400–800 nm range, making it suitable for high-resolution imaging.
No. The platform is designed for pre-formed spheroids or organoids.
We recommend aggregating your 3D model in Akura™ 96 or 384 Microplates following your established protocol. After passing quality control, spheroids/organoids can be transferred into the Akura™ Twin Platform. This approach also supports multi-organ models that require different production times or media.
Transfer can be done manually, semi-automated, or fully automated, with protocols for Opentrons OT-2 available upon request.
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