Gri3D® is a ready-to-use platform for high-throughput and reproducible organoid culture. We demonstrate
that Gri3D® supports the culture of mouse intestinal organoids. The resulting organoids are homogeneously
sized and are confined within the microwells, thus facilitating their imaging. After induction of differentiation,
the organoids show a typical budding morphology and contain differentiated intestinal cells that express
specific markers. Gri3D® provides an ideal tool to standardize organoid cultures.