3D InSight™ | 3D In Vitro MASH Model for Screening and Target Validation

Screening for Metabolic dysfunction-associated steatohepatitis therapies in the only scalable human 3D in vitro MASH model for industry-ready application.

3D In Vitro MASH Model

MASH (Metabolic Dysfunction-associated Steatohepatitis, recently renamed from NASH) is a complex, multi-faceted disease, impacting more than 115 million people worldwide.

InSphero's 3d in vitro MASH model is designed to reflect the complexity of fatty liver disease. Our MASH Screening platform empowers you to simultaneously assess multiple disease parameters, including

  • Steatosis
  • Inflammation
  • Fibrosis

Consequently, you and your team gain a more comprehensive understanding of your asset's impact and efficacy, enabling early-stage decision-making in your MASH research.

Why MASH Screening with InSphero?

Advance your research with our scalable 3D in vitro MASH model for life-changing therapies, improving the lives of patients worldwide.

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Predictive and robust human 3D in vitro MASH model

As complex as necessary and as simple as possible - our research credo at work. Based on co-culturing disease-relevant primary cells, our model accurately mimics MASH

Continuity from screening to mechanistic research

Our MASH model is not only ideal for automated screening, but also perfect for mechanistic research

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Understand the impact of genetic variants (SNPs)

3D InSight™ MASH model is available in multi-donor form or based on single donors, including MASH-relevant SNPs (e.g., PNPLA3, HSD17B13)

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Simultaneously assess hallmarks of MASH and safety

In addition to providing dependable readouts for steatosis, inflammation, and fibrosis, our MASH model also provides valuable information on cytotoxicity and the therapeutic window

Join the MASH CALL Initiative by September 6

Benefit our scalable, reliable, and reproducible 3D in vitro MASH Model for:

  • Increased throughput and automation
  • Reduced costs for a unique solution

Your Success is Our Mission

Schedule a consultation with our experts

Our Process in MASH Screening

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3D InSight™ Human Liver Microtissue formation

Primary human hepatocytes (PHHs), hepatic stellate cells (HSCs), Kupffer cells, and liver endothelial cells (LECs)

Lipid loading

Using an optimized treatment with free fatty acids and elevated sugar concentration

Compound dosing

Precise induction of the desired MASH phenotype followed by compound addition

Multiparametric readouts

Quantification of lipids, inflammation, and fibrosis using biochemical or imaging assays

MASH CALL Initiative | September 2024

MASH CALL - 3d in vitro mash model

Unlock the full potential of MASH Screening

We are pioneering the approach for drug discovery in MASH by launching a CALL with valuable insights into the:

  • Anti-steatotic and anti-fibrotic effects of your compounds
  • Comparison with clinical candidates and reference controls

Unlock the full potential of your research with:

✔️ Increased throughput and automation enabled by scalable 3D in vitro MASH Model
✔️ Reduced costs for a unique solution

Featured resources

A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates
Redefining NASH Drug Discovery: A New Approach to Complex Disease Modeling
3D InSight™ Liver Platform for Liver Disease Research

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