Streamline your research with standardized, ready-to-use, primary cell-based 3D liver models in high-density culture format.
3D InSight™ Species-Specific Liver Microtissues enable direct comparison of hepatotoxicity across human and preclinical species, helping determine whether animal safety signals are relevant to humans and strengthening translational confidence.
Derived from the most widely used IND-enabling species, these physiologically relevant 3D microtissues replicate native liver architecture and function. Each spheroid contains carefully selected, species-specific primary hepatocytes co-cultured with non-parenchymal cells to preserve key hepatic activities.
Delivered assay-ready, 3D InSight™ Liver Microtissues support immediate deployment, enabling consistent, scalable, and reproducible data generation from day one.
3D InSight™ Species-Specific Liver Microtissues are the industry’s only assay-ready solution featuring 96 uniform, fully species-specific co-culture spheroids with up to four weeks of viability. The format is automation-compatible and scheduling-flexible, accelerating high-quality safety assessment and decision-making.
Portfolio of our Assay-Ready 3D InSight™ Species-Specific Liver Microtissues
– 3D InSight™ Beagle Dog Liver Microtissues
– 3D InSight™ C57BL/6 Mouse Liver Microtissues
More preclinical species are available through Liver Safety Services.
Fully primary, species-specific hepatocytes and non-parenchymal cells mimic in vivo liver physiology, delivering more predictive translational data.
Aligned human and non-human platform deliver directly comparable results, streamlining discovery of cross-species differences.
With up to 4 weeks of viability and maintained functionality, our 3D liver models support flexible study designs and timelines- ready for diverse research needs upon delivery.
Produced every three weeks and delivered worldwide, our assay-ready 3D liver microtissues arrive with everything you need to start generating results from day one.
Standardized automated three-weekly production and pharma-grade QC deliver consistent spheroids and reproducible results – ideal for studies at any throughput.
3D InSight™ Species-Specific Liver Microtissues - The only fully species-specific scalable in vitro model
Species-Specific Liver Microtissues produced in 2025
Drug Developers Adopted 3D InSight™ Species-Specific LiMTs in 2025
Industry Partners participated in model validation
Peer-Reviewed Publications
Extend your experimental timelines without compromising data quality. Our 3D InSight™ Species-Specific Liver Microtissues maintain high viability and consistent albumin secretion for up to 4 weeks, enabling reliable long-term and repeated-treatment studies adaptable to your needs.
Figure: Albumin measurement in 3D InSight™ Dog Liver Microtissues in 28 days of culture.
Conduct long-term functional assays confidently using 3D InSight™ Species-Specific Liver Microtissues, which maintain stable cytochrome P450 (CYP) activity for an extended period. This enables robust and accurate assessments in long-term liver safety, efficacy, drug metabolism, or pharmacokinetics studies.
Figure: measurement of metabolite concentration in 3D InSight™ Dog Liver Microtissues after CYP substrate application.
Achieve more accurate predictions with high functional models, suitable for safety assessment of different drug modalities.
Figure: immunohistochemical staining of 3D InSight™ Mouse Liver Microtissues.
Our streamlined automated production process and rigorous pharma-grade quality control ensure consistent, uniform spheroids across batches. Using large, cryopreserved cell lots ensures minimal batch-to-batch variability – so you can confidently plan long-term studies and repeat experiments, knowing you will generate the same high-quality, reproducible data every time.
Figure: Graph showing ATP and diameter values of different 3D InSight™ Dog Liver Microtissues production at day 1 after aggregation
Our species-specific liver microtissues are produced every three weeks.
You can place your order before the order deadline listed in the production schedule.
From order deadline to experiment in 14 days – our 3D liver microtissues arrive assay-ready to fast-track your research from day one.
Order 3D InSightTM Species-Specific Liver Microtissues
InSphero takes care of the production, QC (week 1)
InSphero ships your order in the InFloat™ system with medium (week 2)
Receive our assay-ready 3D Liver models at your doorstep (within week 2)
Species-Specific Liver Microtissues
Dog Liver microtissues:
Mouse Liver microtissues:
Deliverables
Assay-Ready 3D InSight™ Species-Specific LiMTs – Product Manual
In 2D hepatocyte cultures, liver cells are grown as a flat monolayer on a plastic surface. While simple to handle, these cells rapidly lose liver-specific functions, such as cytochrome P450 activity and albumin production, limiting their use for long-term or predictive studies. In contrast, 3D liver spheroids, such as InSphero’s 3D InSight™ Human and Species-Specific Liver Microtissues, are self-assembled co-cultures of hepatocytes and non-parenchymal cells that mimic the structural and functional complexity of the liver.
These 3D models maintain liver-specific functions over several weeks, support long-term and repeated-dose studies, and better replicate the in vivo liver environment, enabling more human-relevant and translational insights.
Once received, the 3D liver microtissues maintain high viability, albumin secretion, and stable CYP450 activity for up to 4 weeks, supporting short- and long-term experiments, including repeated dosing.
Each production batch of assay-ready 3D InSight™ Human and Species-Specific Liver Microtissues is manufactured using a standardized, controlled process to ensure consistency. Every batch undergoes rigorous quality control, including checks for spheroid size, ATP content, and confirmation of one spheroid per well. To support transparency and reproducibility, each plate is shipped with a certificate of analysis (CoA).
They are pre-aggregated, viable 3D liver spheroids composed of species-specific primary hepatocytes and liver non-parenchymal cells (including but not exclusively Kupffer and endothelial cells), delivered in Akura™ 96 spheroid microplates (1 spheroid/well). Ready for immediate use upon arrival. No thawing, seeding, or pre-culture required.
The assay-ready 3D InSight™ Species-Specific Liver Microtissues are delivered in Akura™ 96 spheroid microplates, optimized for 3D spheroid culture, no transfer is needed. To maintain viability and function, you should use the appropriate Species-Specific 3D InSight™ Liver Maintenance Medium (dog TOX, mouse TOX), provided or specified with your order.
Donor lot changes at InSphero are infrequent, thanks to the use of large pooled donor lots, enabling consistent supply for long-term studies. To ensure data consistency, InSphero follows a standardized production process and performs comprehensive lot validation before a donor lot is released for production. Each lot is evaluated for key hepatic and non-parenchymal cell markers (e.g., ASGR1, CK18, BSEP, CD68, α-SMA, CD3), and functionally characterized over time for ATP levels, albumin secretion, cytochrome P450 activity.
Only lots that meet strict performance criteria are approved for production, ensuring high-quality, consistent data across experiments.
Currently, we offer 3D InSight™ Beagle Dog and C57BL/6 Mouse Liver Microtissues as Assay-Ready product. Other preclinical species are available as services performed in our headquarters in Schlieren, ZH, CH.
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