Akura™ Immune Flow Organ-on-Chip Platform
Immune-Competent Microfluidic Multi-Organ Platform
Akura™ Immune Flow Organ-on-Chip Platform
Building on the features and concept of the Akura™ Flow Chip, the Akura™ Immune Flow enables the addition of suspension cells to the multi-organ system. A patented design of the microfluidic channel prevents sedimentation of the cells and enables a continuous interaction of the cells with multiple spheroids and organoids in the system under flow conditions. The Akura™ Immune Flow adds immune competence to multi-organ systems while ensuring a seamless integration and optimal utilization of standardized 3D models.
Discover Akura™ Immune Flow Organ-on-Chip
Microfluidic Chip Design
The Akura™ Immune Flow Chip features two units per chip, each with seven compartments in a central interaction chamber. Microfluidic channels connect the chamber to top and bottom reservoirs, enabling bi-directional flow for dynamic interactions between suspension cells and 3D spheroids/organoids.
Modular Standardized Formats
Up to four Akura™ Immune Flow Chips can be organized within a handling frame that adheres to the SLAS/ANSI format. This standard ensures easy integration with existing lab equipment and automation tools, streamlining the platform’s use in high-throughput settings.
Continuous Gravity-Driven Perfusion
The Akura Flow Programmable All-In-One Tilter supports simultaneous culture of up to four frames. Its unique meandric channel design and vertical tilting prevent cell sedimentation by resuspending settled cells through 170° tilting, allowing them to naturally resettle as the medium flows.
User-Friendly Operation
The ULA coating on the chip’s compartments and microchannels preserves spheroid/organoid integrity and minimizes cell adhesion during long-term culture. Wide top-access ports enable easy loading and retrieval, while the flat, transparent COC bottom ensures clear imaging. Large reservoirs allow efficient medium exchange and sampling.
Rely on an innovative perfusion concept for suspension cells
- Patent-pending, meandric channel combined with vertical orientation prevents cell sedimentation and keeps cells in suspension
- Cell interaction chamber with a large channel reduces flow speed and enhances long-term interactions
- Gravity-based flow with defined medium volume enables multi-passage of cells and secretome analysis and effects
Experience an intuitive and robust handling
- Standard chip format and parallelization by assembly in ANSI/SLAS handling frame for quick implementation with widely used lab tools
- Conical well design with SureXchange™ ledge for easy loading and access of spheroids
- Pump and tube-free perfusion for flexible scale up and access to medium and cells
Access a wide range of readout methods:
- Anytime access to supernatants using manual or automated pipetting
- Retrieval of spheroids for lytic assays, histology or RNA sequencing
- In-situ high-content imaging with minimal optical aberration with a flat and transparent COP bottom
Applications of Akura™ Immune Flow Organ-on-Chip Platform
- Investigate interaction of cells in flow with multiple static spheroids
- Immune cell adhesion and invasion
- Interaction of circulation tumor cells with different organs to investigate their metastatic potential.
Akura™ Immune Flow Organ-on-Chip Starter Kit
Bring the Akura™ Immune Flow Organ-on-Chip System into your lab by acquiring our starter kit offering. It’s everything you need to immediately begin investigating complex biological questions in your research:
Akura™ Flow Programmable All-In-One Tilter
Controls gravity-based flow in incubator with intuitive built-in user interface
Akura™ Immune Flow Kit (2/pack)
2x frames, each with 4 chips and supplements to run an experiment with up to 16 experimental conditions
Akura™ Immune Flow Refill (2/pack)
8 chips and supplements to run an experiment with up to 16 conditions.
Akura™ Tilting Stand
Simple handling tool providing gravity-based flow while performing medium exchange under the hood.
20x Pack of Akura™ 96 Microplate
For 3D spheroid aggregation before transferring into Akura™ Immune Flow Organ-on-Chip or for collection after experiments and downstream analysis.
1h Akura™ e-Introduction
Learn about concept, design and characteristics of the Akura™ Immune Flow OoC Platform.
Akura™ Organ-on-Chip Training & Support
Personal support and consulting to set up the first experiments with InSphero’s scientific experts.
Akura™ Organ-on-Chip Technology Access Package
Allows for discounted Refills & Voucher (2000 CHF) for InSphero products (valid 6 months)
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