3D InSight™ Human Liver Microtissues, Human Liver Models | InSphero

Closely approximate the human response to compound exposure

3D InSight™ Human Liver Microtissues are pharma-validated, preclinical co-culture liver models for toxicology testing that have been shown to achieve 2X higher sensitivity for DILI prediction compared to traditional 2D PHH.  These advanced 3D liver models, which combine multi-donor diversity with structural and functional robustness, are ideal for drug safety and efficacy testing as well as the study of healthy and diseased liver function. Upon request, microtissues from individual donor hepatocyte lots are also available.

  • Reflect the average response of a population with a model incorporating hepatocytes from 5 male and 5 female donors
  • Achieve the highest biological relevance in an in vitro model with primary hepatocyte, Kupffer cell, and liver endothelial cell (LEC) co-cultures
  • Test long-term effects of drug exposure in an organotypic, functional liver model
Human liver spheroid model for tox testing

Characterization Data

Human Liver Microtissue Specifications

  • PHH from pooled male/female donors
  • Co-culture with Kupffer cells and LECs
  • Guaranteed 28 days viability
  • 96-well format, 1 microtissue per well
  • 384-well format coming soon
  • Biweekly production

Order Information

Catalog #Description
Microtissues
MT-02-302-043D InSight™ Human Liver Microtissues (multi-donor hepatocytes, co-culture with Kupffer cells and LECs), 96x
MT-02-302-013D InSight™ Human Liver Microtissues (multi-donor hepatocytes, monoculture), 96x
MT-02-002-043D InSight™ Human Liver Microtissues (single-donor hepatocytes, co-culture with Kupffer cells and LECs), 96x
MT-02-002-013D InSight™ Human Liver Microtissues (single-donor hepatocytes, monoculture), 96x
Kits
AK-01-001-013D InSight™ Cell Viability Assay Kit for up to 4x 96-well plates
Media*
CS-07-001-01Maintenance Medium – TOX*
(for tox testing), 500 mL
CS-07-001a-01Maintenance Medium – AF*
(for basic cultivation), 500 mL
CS-07-001b-01Maintenance Medium – INF*
(for inflammatory tox testing), 500 mL

* only available when purchased with 3D InSight™ Microtissues

Request Additional Resources

Brochure: Human Liver Microtissues

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Publication: Proctor et al. (Arch Toxicol, 2017)

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Poster: Multi-donor Human Liver Microtissues

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Find out how 3D InSight™ Liver Microtissues can provide greater

confidence in safety testing of your drugs.

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